Exploration of the phase diagram of liquid water in the low-temperature metastable region using synthetic fluid inclusions.

We present new experimental data of the low-temperature metastable region of liquid water derived from high-density synthetic fluid inclusions (996-916 kg m-3) in quartz. Microthermometric measurements include: (i) prograde (upon heating) and retrograde (upon cooling) liquid-vapour homogenisation. We used single ultrashort laser pulses to stimulate vapour bubble nucleation in initially monophase liquid inclusions. Water densities were calculated based on prograde homogenisation temperatures using the IAPWS-95 formulation. We found retrograde liquid-vapour homogenisation temperatures in excellent agreement with IAPWS-95. (ii) Retrograde ice nucleation. Raman spectroscopy was used to determine the nucleation of ice in the absence of the vapour bubble. Our ice nucleation data in the doubly metastable region are inconsistent with the low-temperature trend of the spinodal predicted by IAPWS-95, as liquid water with a density of 921 kg m-3 remains in a homogeneous state during cooling down to a temperature of -30.5 °C, where it is transformed into ice whose density corresponds to zero pressure. (iii) Ice melting. Ice melting temperatures of up to 6.8 °C were measured in the absence of the vapour bubble, i.e. in the negative pressure region. (iv) Spontaneous retrograde and, for the first time, prograde vapour bubble nucleation. Prograde bubble nucleation occurred upon heating at temperatures above ice melting. The occurrence of prograde and retrograde vapour bubble nucleation in the same inclusions indicates a maximum of the bubble nucleation curve in the ϱ-T plane at around 40 °C. The new experimental data represent valuable benchmarks to evaluate and further improve theoretical models describing the p-V-T properties of metastable water in the low-temperature region.

Ciliary beating plane and wave propagation in the bovine oviduct.

The uterine tube is an essential conduit for the gametes and zygote during reproduction. The necessary bidirectional conveyance occurs through peristalsis and ciliary activity, but unlike in respiratory tract, little is known about mucociliary transport in the uterine tube, and the direction of transport and the alignment of oviductal cilia have not been conclusively characterized. This study aimed to determine the uniformity in the axonemal orientation of motile cilia in the bovine uterine tube, to identify the direction of mucociliary transport and to relate the presumptive beating plane and the mucociliary transport direction to the long axis of the uterine tube. The angular spread of oviductal motile cilia was determined by electron microscopy, and by maintaining the accurate alignment of the samples throughout the processing steps, axonemal orientation was determined relative to the long axis of the oviduct. The direction of the effective mucociliary transport was determined by the analysis of video microscopic data recorded on explants. Vector-based analysis of electron micrographs yielded the mean angle of deviation between the 'effective ciliary stroke', as derived from axonemal orientation, and the tubal longitudinal axis pointing towards the uterus to be 0.8°, with a standard deviation of 35.2°. The corresponding angular deviation of the short-wave propagation was -6.8° (SD 34.6°). These results show that oviductal motile cilia are rigorously aligned, that the beating plane of the cilia is parallel to the long axis of the uterine tube and that the 'effective stroke' and mucociliary transport are directed towards the uterus.

Functional imaging of mucociliary phenomena: high-speed digital reflection contrast microscopy.

We present a technique for the investigation of mucociliary phenomena on trachea explants under conditions resembling those in the respiratory tract. Using an enhanced reflection contrast, we detect simultaneously the wave-like modulation of the mucus surface by the underlying ciliary activity and the transport of particles embedded in the mucus layer. Digital recordings taken at a speed of 500 frames per second are analyzed by a set of refined data processing algorithms. The simultaneously extracted data include not only ciliary beat frequency and its surface distribution, but also space-time structure of the mucociliary wave field, wave velocity and mucus transport velocity. Furthermore, we propose the analysis of the space and time evolution of the phase of the mucociliary oscillations to be the most direct way to visualize the coordination of the cilia. In particular, this analysis indicates that the synchronization is restricted to patches with varying directions of wave propagation, but the transport direction is strongly correlated with the mean direction of waves. The capabilities of the technique and of the data-processing algorithms are documented by characteristic data obtained from mammalian and avine tracheae.

Noise on Fluorescence Correlation Spectroscopy.

The time dependence of the noise and the signal-to-noise (SN) ratio of the fluorescence correlation spectroscopy (FCS) autocorrelation function is obtained from replica measurements of standard dextran solutions. The noise dependence on the delay time is fitted by a hyperbolic function with two fitting parameters. The dependence of these parameters on concentration, fluorescence intensity, and accumulation time is obtained experimentally. The behavior of SN at zero delay time agrees well with the theoretical predictions reported in the literature. The obtained data are useful for the quantitative evaluation of the FCS data fits, as well as for simulation of the FCS autocorrelation functions. Copyright 2001 Academic Press.

[Protein-condensation diseases--molecular basis of cataract formation]. / Protein-Kondensationskrankheiten--Molekulare Mechanismen bei der Kataraktogenese.

There are reasons to classify a number of apparently disparate diseases as "condensation" (or molecular aggregation) diseases. Examples of such condensation diseases include the late phase of diabetes mellitus, Alzheimer's disease and others. With an expanding knowledge, the list of these diseases is likely to increase. We shall describe the underlying common mechanisms, the aim being to find anticataractogenic drugs based on this insight. The common, most important denominator of various clinically differing condensation diseases derives from the interaction of the macromolecules which is in part attractive and in part repulsive. Aggregation resp. clumping of the macromolecules of the crystalline lens, the reasons for light scattering, may be prevented by introducing a number of molecules of various designs into the original macromolecular complex which reduce the tendency of aggregation. Cataract inhibitors of this category may be regarded as effective if they are able to increase the time constant of the normal aging process (i.e. the increment of scatter) by about 20%.

Colloidal Particles at Water-Glass Interface: Analyzing Videomicroscopic Data.

Direct videomicroscopic observations provide a powerful tool for investigations on the deposition of colloidal particles at liquid-solid interfaces. However, the technique is also capable of producing artefacts caused mainly by limited resolution. In the present contribution we discuss the possibilities and limitations of videomicroscopic observations, focussing thereby on an application example, namely particle deposition from flow in a parallel plate channel in the presence of a repulsive barrier. We outline algorithms for the determination of the relevant quantities, indicate the pitfalls, and provide correction formulas. Special attention is paid to the kinetics of particle release, namely to the accurate determination of the distribution of the times the particles spend adhering to the surface. In our example the kinetics of the release is found to be highly nonexponential, but an adequate fit of the measured distribution of adhesion times is obtained with a stretched exponential exp[-(betatau)nu], where nu approximately 0.5 and beta approximately 3 x 10(-5) s-1. Copyright 1998 Academic Press.

Colloidal Particles at Water-Glass Interface: Deposition Kinetics and Surface Heterogeneity.

Videomicroscopy in combination with evanescent field illumination is applied to study the sorption of colloidal particles from flow in a parallel plate channel on a glass surface. The experiments, carried out in the presence of a repulsive electrostatic barrier, reveal surprisingly complex results: The glass surface, though optically flat and well cleaned, is not homogeneous, but rather the sorption occurs at a limited number of preferred sites. Moreover, these sites are not static: new sites keep appearing at random positions on the observed surface and disappearing at a rate of kd = 1.3 x 10(-5) s-1. These findings can be understood within a simple model that takes into account slow but inevitable dissolution of the glass surface. The bulk glass contains potential adsorbers, which are continuously being exposed by the dissolution process and act as transient adsorption sites, before being washed off by the flowing buffer solution. Copyright 1998 Academic Press.

Colloidal Particles at Solid-Liquid Interfaces: Mechanisms of Desorption Kinetics.

We study the sorption of colloids on equally charged surfaces. Our focus is on the time scale from hours to weeks, where adsorption is not an irreversible process but interplays with (spontaneous) desorption. Using model calculations, we show how the desorption kinetics is influenced by readsorption, a potential barrier, a secondary potential minimum, local variation of the potential, and bond aging. In the experimental part we present results of in situ observation of the sorption kinetics of polystyrene latex particles onto a glass surface. Combining the evanescent field method with video microscopy, we were able to identify the particle arrival and departure times individually and therefrom determine the adhesion time distribution function. The nonexponentiality of this function can be explained by a gamma distribution of the potential depth at the binding sites as well as by logarithmic bond aging. Copyright 1998 Academic Press.

Mode-selective dynamic light scattering: theory versus experimental realization.

We present a quantitative experimental comparison of fiber-based, single- and few-mode dynamic light scattering with the classical pinhole-detection optics. The recently presented theory of mode-selective dynamic light scattering [Appl. Opt. 32, 2860 (1993)] predicts a collection efficiency and a signal-tobaseline ratio superior to that of a classical pinhole setup. These predictions are confirmed by our experiments. Using single-mode optical fibers with different cutoff wavelengths and commercially available mechanical components, we have constructed a mode-selective detection optics in a simple and compact dynamic light-scattering spectrometer that permits an optimal compromise between signal intensity and dynamical resolution.

High-resolution distributed temperature sensing with the multiphoton-timing technique.

We report on a multiphoton-timing distributed temperature sensor (DTS) based on the concept of distributed anti-Stokes Raman thermometry. The sensor combines the advantage of very high spatial resolution (40 cm) with moderate measurement times. In 5 min it is possible to determine the temperature of as many as 4000 points along an optical fiber with an accuracy Δ T < 2 °C. The new feature of the DTS system is the combination of a fast single-photon avalanche diode with specially designed real-time signal-processing electronics. We discuss various parameters that affect the operation of analog and photon-timing DTS systems. Particular emphasis is put on the consequences of the nonideal behavior of sensor components and the corresponding correction procedures.

Dynamic light scattering with single-mode and multimode receivers.

Single-mode optical fibers provide the ideal receiver optics for dynamic light-scattering measurements. Theoretical analysis shows that with a single-mode fiber one can achieve a theoretical limit of 1 for the coherence factor while maintaining a high light-collection efficiency. In fact, the sensitivity of the single-mode receiver surpasses that of a classical two-pinhole setup with a coherence factor of 0.8 by a factor of 4 and the advantage increases rapidly when a still higher coherence factor is desired. In addition, a single-mode fiber receiver offers the possibility of working with an arbitrary large scattering volume and with an arbitrary working distance. All these features are also demonstrated experimentally by a remarkably simple apparatus that consists, essentially, of a commercial laser beam delivery assembly.